Brassica

genetics for the classroom

Mutant Library
Wild-type

Seedling
Albino
Altered Cotyedon Shape
Long Hypocotyl
Multicotyledon
Striped Cotyledons

Plant Shape
Bushy
Dwarf
Dwarf-like
No Bolt
Short

Stem Color
Albino Stem

Flower Shape
Apetala
Trumpet Shaped Petals

Flower Color
Yellow

Leaf Color
Yellow green
Glossy
Pale
V
eriagated
White Tip

Leaf Shape
Wrinkly
Bumpy
Curly
Altered Leaf Shape

Other
Male Sterile
Late Flowering
Lesion Mimic

Mutagenesis

Dictionary of terms

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The principal investigators for this project are:
Richard M. Amasino and Thomas Osborn.

Copyright 2002
University of Wisconsin

Questions or comments about this site should be sent to the webmaster

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Wisconsin rapid cycling Brassica oleracea mutagenesis

wild type Brassica oleraceaPlants are a cost effective, easy and exciting way to bring genetics and developmental biology into the classroom, and to provide students a hands-on opportunity to learn these areas.

Brassica oleracea is a species that comes in many forms. Cabbage, broccoli, and cauliflower are all varieties of Brassica oleracea. Another variety is a small, hearty, fast-growing plant. Our goal is to bring genetics to life in the classroom with this fast-growing variety. In order to create plants that are interesting for students to study, eight thousand of these fast-growing Brassica oleracea plants were mutagenized with EMS, a chemical that causes base changes in the plant's DNA. The resulting population was screened for plants that looked visibly different from their parents. Some of these changes include different colored flowers, different colored leaves, dwarfed plants, plants that lack petals, and plants that have an overall different shape. A few of the changes are illustrated below; the original plant part, or wild-type, is shown on the left while the mutant is shown on the right.

eh635 321 adult_wt_stem_&_eh20_4 adult_wt_&_342i

This work has been funded by the United States Department of Agriculture